Journal: Cancers

Article Title: The Thermal Dose of Photothermal Therapy Generates Differential Immunogenicity in Human Neuroblastoma Cells

doi: 10.3390/cancers14061447

Figure Lengend Snippet: PBNP-PTT generates a thermal dose window of immunogenic cell death in SH-SY5Y and LAN-1 cells in vitro. Three million ( A – C ) SH-SY5Y or ( D – F ) LAN-1 cells were exposed to various thermal doses using PBNP-PTT. After 24 h, cells were analyzed for ( A , D ) intracellular ATP, ( B , E ) HMGB1 release, and ( C , F ) surface calreticulin expression, represented as median fluorescence intensity (MFI). Inset values in the histograms denote the thermal dose. The extent of ICD as measured by its correlates is more pronounced in SH-SY5Y cells compared with LAN-1 cells. Ordinary one-way ANOVA was used to calculate significance between vehicle and different thermal doses and laser alone for HMB1 analysis. n = 2/group; * p < 0.03, ** p < 0.002, *** p < 0.0002, **** p < 0.0001.

Article Snippet: After 24 h incubation at 37 °C post-PBNP-PTT, cells were harvested and stained with Zombie Aqua Fixable viability dye (Biolegend, #423102), blocked with human TruStain Fc block (Biolegend, #422302), and stained with fluorescent antibodies against calreticulin (Abcam, #ab83220), CD80 (Biolegend, #305238), CD86 (Biolegend, #305428), PD-L1 (Biolegend, #329714), B7-H3 (Biolegend, #351010), HLA-ABC (Biolegend, #311432), HLA-DR (Biolegend, #307633), PVR (Biolegend, #337628), and GD2 (Biolegend, #357308).

Techniques: In Vitro, Expressing, Fluorescence

Journal: Cancers

Article Title: The Thermal Dose of Photothermal Therapy Generates Differential Immunogenicity in Human Neuroblastoma Cells

doi: 10.3390/cancers14061447

Figure Lengend Snippet: PBNP-PTT triggers greater immunophenotypic changes in MYCN-non-amplified SH-SY5Y cells than MYCN-amplified LAN-1 neuroblastoma cell line in vitro. SH-SY5Y (blue) and LAN-1 (green) cells were treated with varied thermal doses via PBNP-PTT and analyzed for ( A ) % live cells ( B ) intracellular ATP, ( C ) secreted HMGB1, and cell surface expression levels of ( D ) calreticulin, ( E ) CD80, ( F ) CD86, ( G ) PD-L1, ( H ) B7-H3, ( I ) HLA-ABC, ( J ) HLA-DR, ( K ) PVR, and ( L ) GD2. Data represent mean ± SD ( n = 2 independent samples).

Article Snippet: After 24 h incubation at 37 °C post-PBNP-PTT, cells were harvested and stained with Zombie Aqua Fixable viability dye (Biolegend, #423102), blocked with human TruStain Fc block (Biolegend, #422302), and stained with fluorescent antibodies against calreticulin (Abcam, #ab83220), CD80 (Biolegend, #305238), CD86 (Biolegend, #305428), PD-L1 (Biolegend, #329714), B7-H3 (Biolegend, #351010), HLA-ABC (Biolegend, #311432), HLA-DR (Biolegend, #307633), PVR (Biolegend, #337628), and GD2 (Biolegend, #357308).

Techniques: Amplification, In Vitro, Expressing

Journal: The Journal of Physiology

Article Title: Exercise training alters skeletal muscle microvascular endothelial cell properties in recent postmenopausal females

doi: 10.1113/jp286269

Figure Lengend Snippet: Figure 4. Endothelial cell expression of oestrogen receptor and oestrogen-related receptor content pre- and post-8 weeks of intense aerobic exercise training in recent and late postmenopausal females A–E, ERα protein (A), ERβ (B), ERα/ERβ ratio (C), GPER protein (D) and ERRα protein (E) pre- (light coloured bars) and post- (dark coloured bars) exercise training in recent (RPF; n = 11; grey bars) and late (LPF; n = 11; rose bars) postmenopausal females. F, representative blots and stain-free images for the proteins. For ERα, ERβ, ERα/ERβ ratio, GPER and ERRα one subject from the LPF was missing due to lack of sample. Data are presented as means and individual values. # denotes a difference between groups at the specific time point; ‡ denotes an inter- action between time and group: B, ‡P = 0.034; C, ‡P = 0.020; D, #P = 0.028. [Colour figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Primary antibodies for ERα (8644, Cell Signaling Technology, Danvers, MA, USA; RRID:AB_2617128; 1:1000; 5% milk), ERβ (Mab-7106; R&D Systems, Minneapolis, MN, USA; RRID:AB_10971825; 1:500; TBST), ERRα (76228; Abcam, Cambridge, UK; RRID:AB_310325; 1:750; 2% milk), G protein-coupled oestrogen receptor (GPER; 39742; Abcam; RRID:AB_1141090; 1:500; 5% milk), endothelial nitric oxide synthase (eNOS; 5589; Abcam; RRID:AB_304967; 1:1000; 5% fish gelatin), prostacyclin synthase (PGI2S; 23668; Abcam; RRID:AB_2300427;1:300; 3% BSA), superoxide dismutase (SOD2; 06-984; Merck; RRID:AB_310325; 1:5000; 2% milk), NADPH oxidase 2 (NOX2, gp91phox; 129068; Abcam; RRID:AB_11144496; 1:3000; 3% BSA), citrate synthase (CS; 96600; Abcam; RRID:AB_10678258; 1:500; 3% BSA), glutathione peroxidase 1 (GPX1; 22604; Abcam; RRID:AB_2112120; 1:500; 5% milk) and phosphofructokinase-1 (PFK1; 166722; Santa Cruz Biotechnology; RRID:AB_2236976; 1:2000; 3% BSA) were run.

Techniques: Expressing, Staining

Journal: Fluids and Barriers of the CNS

Article Title: Structural characterization of SLYM—a 4th meningeal membrane

doi: 10.1186/s12987-023-00500-w

Figure Lengend Snippet: Primary antibody information

Article Snippet: Fibroblast Antibody (ER-TR7) , Isolated C3H thymic stromal cells , Novus (NB100-64932) , Rat IgG2a , 1:300 , AB_963381 , .

Techniques: Recombinant, Isolation

Journal: PLoS ONE

Article Title: Maternal Nicotine Exposure Leads to Impaired Disulfide Bond Formation and Augmented Endoplasmic Reticulum Stress in the Rat Placenta

doi: 10.1371/journal.pone.0122295

Figure Lengend Snippet: Forward and reverse sequences for the primers used for quantitative Real-Time PCR.

Article Snippet: KDEL (Grp78) (10C3) , Mouse monoclonal , 1:300 , Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA (#sc-58774).

Techniques:

Journal: PLoS ONE

Article Title: Maternal Nicotine Exposure Leads to Impaired Disulfide Bond Formation and Augmented Endoplasmic Reticulum Stress in the Rat Placenta

doi: 10.1371/journal.pone.0122295

Figure Lengend Snippet: Western Blot primary antibodies, dilutions used in experiments, and company and catalogue information.

Article Snippet: KDEL (Grp78) (10C3) , Mouse monoclonal , 1:300 , Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA (#sc-58774).

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Maternal Nicotine Exposure Leads to Impaired Disulfide Bond Formation and Augmented Endoplasmic Reticulum Stress in the Rat Placenta

doi: 10.1371/journal.pone.0122295

Figure Lengend Snippet: Protein and mRNA levels of targets of interest were determined via Western blot and RT-PCR, respectively. (A) Specific targeted protein bands as detected by respective antibodies via Western blot. (B) Atf4 mRNA levels. (C) Atf4 protein levels. (D) Grp78 mRNA levels. (E) Grp78 protein levels. (F) CHOP mRNA levels. (G) CHOP protein levels. All protein levels were expressed as means normalized to β-Actin ± SEM (n = 5-6/group). All mRNA levels were expressed as means normalized to the geometric mean of three stable housekeeping genes (β-Actin, 18S, and Gapdh) ± SEM (n = 5-6/group).*, Significant difference (p < 0.05). **, Significant difference (p<0.01).

Article Snippet: KDEL (Grp78) (10C3) , Mouse monoclonal , 1:300 , Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA (#sc-58774).

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: The Journal of cell biology

Article Title: Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads.

doi: 10.1083/jcb.202103003

Figure Lengend Snippet: Figure 1. Lumen morphogenesis in hepatocytes is accompanied by specific actin structures that interconnect the two lumen-forming cells. (a) Schematic overview of primary Dlk1+ hepatoblasts in culture differentiating into hepatocytes and recapitulating BC formation. (b) Differentiated hep- atocytes form branched interconnected BC lumina. Immunofluorescence microscopy images of differentiated hepatocytes (Protocol 2, Materials and methods) stained for F-actin with phalloidin–Alexa 488, and for apical markers CD13 and ZO-1. Scale bar: 10 µm. (c) In vitro differentiated (Diff.) hepatocytes express

Article Snippet: The primary antibodies rat monoclonal anti-CD13 (Novus; NB100-64843; RRID:AB_959651; 1:500) and rabbit monoclonal anti-Sox9 (clone EPR14335-78; Abcam; cat. no. ab185966; RRID:AB_2728660; 1: 500) were diluted in Tx buffer (0.2% gelatin, 300 mMNaCl, and 0.3% Triton X-100 in PBS) and incubated for 2 d at RT.

Techniques: Immunofluorescence, Microscopy, Staining, In Vitro